Effect of Vancomycin on Polymicrobial Biofilm:Staphylococcus aureus and Candida albicans
B.Vinod Kumar
Assistant professor, Dept. of Microbiology, Faculty of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia
Abstract:
S.aureus does not form biofilm readily on abiotic surfaces where as C.albicans forms readily biofilm on indwelling medical devices. It is reported that eight percent of blood stream infections were polymicrobial. Twenty seven percent of nosocomial C.albicans blood stream infections are polymicrobial, as the third most common polymicrobial isolates were C.albicans with S.aureus. Hence in the present study we evaluated the formation of biofilm of S.aureus with C.albicans. S.aureus alone could not form biofilm with in 24 h. of incubation at 370C. S.aureus grown along with C.albicans formed biofilm within 24 h of incubation at 370C. We also tested whether the S.aureus is sensitive to vancomycin in the presence of C.albicans. In our study we found that S.aureus was sensitive to vancomycin at concentration of 2.5 micrograms/ml in the presence of C.albicans. Vancomycin (2.5 micrograms/ml) is effective in eradicating biofilm formation of S.aureus even in presence of C.albicans.
